Compare to previous studies10, 11, 12, 13, 17, 18, we replicated the association between DNA methylation level and obesity at 10 gene loci (ANKRD11: ankyrin repeat domain 11, AVPI1: arginine vasopressin-induced 1, CDK19: cyclin-dependent kinase 19, FOXK2: forkhead box K2, HDAC4: histone deacetylase 4, IFT140: intraflagellar transport 140, KIAA0753, LTBP1: latent transforming growth factor beta binding protein 1, MYOM2: myomesin 2, TBC1D8: TBC1 domain family, member 8). The gene discussed is LTBP1; the disease is obesity due to melanocortin 4 receptor deficiency.