Similar to our study, decreased M1 and unchanged M2 marker expression were obtained in perivascular fat of ApoE−/− mice housed at 22°C compared to 30°C; however, these mice did not show a difference in insulin resistance, but progression of atherosclerosis, a chronic inflammatory disease, was lower in the 22°C mice (Tian et al., 2016). This evidence concerns the gene INS and atherosclerosis.