In contrast, levels of TNF, the IL-12p70 heterodimer, and the IL-12p40 subunit were significantly reduced in the BALF at 24 hours post-infection in anti-Gr-1-treated mice (Fig 2D, 2F and 2G), in agreement with a previous study using anti-Gr-1-mediated depletion during pulmonary L. pneumophila infection [46]. This evidence concerns the gene TNF and infection.