TP53BP1 and cytomegalovirus infection: First, while the overall cellular intensity of 53BP1 staining increased in a similar manner as in the infected BJ cells (compare Fig. 8J and 8 D), the physiological nuclear localization of 53BP1 remained unaltered in the DAOY cells regardless of the HCMV infection, even in the nuclei with strong IE72 expression by 96h postinfection.