PIN1 and hepatocellular carcinoma: To demonstrate the clinical significance of miR-140-5p-mediated Pin1 regulation in HCC, we first determined expression levels of miR-140-5p and Pin1 protein by qRT-PCR and Western blot assay, respectively, in normal human liver cell line LO2 and 7 human HCC cell lines HepG2, Huh7, Hep3B, PLC/PRF/5, MHCC-97H, HCCLM3, SMMC-7721.