AJAP1 and hepatocellular carcinoma: To investigate the effects of AJAP1 on HCC cell proliferation and tumorigenicity, the less invasive SMMC7221 and Huh7 and highly invasive HCCLM3 and SK-Hep-1 cell lines were infected with a lentivirus (LV) expressing short hairpin (sh)RNA targeting AJAP1 (shAJAP1) or encoding AJAP1, respectively.