PRKN and Parkinson disease: AIMP2 accumulation is strongly associated with dopaminergic cell death in PD.9, 10, 11, 12 To identify the potential role of VPS35 in regulating pathogenic parkin substrate AIMP2, we performed a small-scale screening in SH-SY5Y cells transiently transfected with V5-WT VSP35 or the PD-linked mutant version V5-D620N VPS35.