To address the metastasis-associated features of CRC cells regulated by MMP14 and ACTG1, pcDNA3-ACTG1-myc (ACTG1) and pcDNA3-MMP14 (MMP14) plasmids were constructed to intensify MMP14 and ACTG1 expression, and si-MMP14 1#, si-MMP14 2#, si-ACTG1 1# and si-ACTG1 2# siRNAs were synthesized to silence the endogenous MMP14 and ACTG1. The gene discussed is MMP14; the disease is colorectal carcinoma.