We found that CASP1 expression is positively associated with pathways including cytokine–cytokine receptor interaction in LUAD, BRCA and LUSC, and metabolic pathways in COAD, but negatively associated with pathways, such as tight junction in LUAD, endocytosis, AMPK and mTOR signaling in BRCA and COAD (Supplementary Figure S3). The gene discussed is CASP1; the disease is colon adenocarcinoma.