CASP1 and colon adenocarcinoma: To investigate the biological function of CASP1 in tumorigenesis, we carried out bioinformatics analysis to examine the cellular pathways either positively or negatively correlated with the CASP1 expression in four different cancer types, including LUAD, breast cancer (BRCA), colon adenocarcinoma (COAD) and lung squamous cell carcinoma (LUSC) from the TCGA database (Supplementary Figure S3).