To assess the effect of dual inhibiting OCT4 and AKT on cancer cells of somatic origin, we chose two human glioblastoma cell lines (U87 and U251) that are known to express low levels of OCT419, 20, infected them with sh-OCT4, and then treated them with Akti-1/2. The gene discussed is POU5F1; the disease is glioblastoma.