HMGB1 and ischemia: After 60 min of ischemia and 6 h of reperfusion, the expression of HMGB1 in both the PBS-treated and scrambled siRNA-treated control groups was markedly attenuated, as compared to 0 h after reperfusion (Fig. 5A); this decrease may have been the result of the aggravated disruption of hepatocytes caused by reperfusion injury.