INS and hyperinsulinism: As predicted, by 24 weeks, HFD-fed mice exhibited weight gain, elevated fasting blood glucose, hyperinsulinemia, and impaired glucose tolerance compared with standard diet-fed controls, while dietary reversal mice exhibited significant weight loss, with improvements in fasting blood glucose and insulin levels, and impaired glucose tolerance when compared with age-matched HFD-fed controls (Fig. 4B-F).