There was no significant correlation between the expression of AFAP1-AS1 or PD-1 and patients' gender, age at diagnosis, tumor size (T stage), lymphatic invasion (N stage), distant metastasis at diagnosis (M stage) and overall clinical staging (Supplementary Table 2), but patients with high expression of AFAP1-AS1 or PD-1 in NPC-infiltrating lymphocytes were more likely to have distant metastasis when they relapsed (Figure 3A and 3B, P=0.005 and P=0.020, respectively). This evidence concerns the gene AFAP1 and nasopharyngeal carcinoma.