Patients with positive expression of AFAP1-AS1 or PD-1 in NPC biopsies had a poor prognosis, with shorter overall survival (47.7% five-year survival with positive expression of AFAP1-AS1 vs. 92.8% with negative expression, P=0.001, Figure 4A; 52.8% five-year survival with positive PD-1 expression vs. 65.0% with negative expression, P=0.049, Figure 4B). The gene discussed is AFAP1; the disease is nasopharyngeal carcinoma.