GPC3 and hemoglobin measurement: The AUROC value, sensitivity and specificity of GPC3 for pretreatment HB versus all controls were 0.63 (95% CI: 0.49 to 0.77), 54.5% and 66%, respectively, at a cutoff of 1.74 ng/mL, all significantly lower than those of AFP (0.91 (95% CI: 0.84 to 0.97), 95.5% and 76.0%, respectively) at a cutoff of 1130 ng/ml) (Fig. 3A).