To further compare the performance of PLA-flow with RQ-PCR, and to see how well suited PLA-flow is for MRD quantification in a routine setting, we assessed an extended series of 81 samples from 36 CML patients, all positive for the BCR-ABL1 major variant, both newly diagnosed (n = 7) and patients that at the time of sampling were under treatment with TKIs, such as imatinib, dasatinib, nilotinib and ponatinib, or had previously undergone TKI treatment (n = 29; Supplementary Table S2). This evidence concerns the gene ABL1 and chronic myelogenous leukemia, BCR-ABL1 positive.