Captured cells were stained with fluorescently labelled anti-CD138 antibody (red), followed by fixing/permeabilization and intracellular staining with either λ or κ light chain antibody (green); representative image of CD138+ λ+, and CD138+ λ- cells from a MM patient bone marrow sample are shown in Fig. 4a. The gene discussed is SDC1; the disease is Miyoshi myopathy.