Also, a study on the expression of cardiac titin in patients with dilated cardiomyopathy reported alterations at the isoform ratio level favouring the more compliant N2BA isoform, with a consequent decrease in passive myocardial stiffness (Nagueh et al. 2004; Gigli et al. 2016) (Fig. 5b). Here, TTN is linked to dilated cardiomyopathy.