We found altered CpG methylation in MD F1 mice (Figure 3; two-way ANOVA with paternal diet as between-subjects factor and CpG position as within-subjects factor: effect of paternal diet, P=0.0896; effect of CpG position, P<0.0001; paternal diet x CpG interaction, P=0.0026), indicating that differential methylation of Kcnmb2 regulatory regions could underlie altered Kcnmb2 expression in MD F1 mice. The gene discussed is KCNMB2; the disease is Menkes disease.