These behavioral and neurophysiological changes in MD F1 mice were associated with altered hippocampal gene expression, including the expression of Kcnmb2—encoding a BK channel subunit—and Mat2a—encoding a methionine adenosyltransferase, as well as promoter methylation changes around the TSS of Kcnmb2. The F2 offspring of MD fathers, in contrast, did not show obvious behavioral differences to controls and displayed normal Mat2a and Kcnmb2 expression, suggesting that the paternal exposure to the MD affected the subsequent generation but left F2 offspring unaffected (but see below). Here, KCNMB2 is linked to Menkes disease.