Patient B393, with an ECHO diagnosis of TOF, carried the JAG1(c.3038A>T, p.H1013L) and GATA6(c.972C>G, p.H324Q) variants; patient B294, with TOF, carried the JAG1(c.2906T>C, p.M969T) and GATA5(c.274G>T, p. A92S) variants; and patients B445 and B548, both of which were diagnosed with TOF and PFO, carried two GATA4 variants (c.1220C>A, p.P407Q;c.1138G>A, p.V380 M). This evidence concerns the gene GATA5 and Patent foramen ovale.