We utilized a panel of prostate cancer cell lines (androgen receptor positive (LNCaP), androgen receptor negative (DU‐145 and PC3)), noncancerous immortalized prostate cell lines (RWPE‐1 and 957E/hTERT), and a noncancerous immortalized nonprostate line (HEK293) to understand the molecular pathways that NDRG1 regulates to elicit its metastasis suppressor function. This evidence concerns the gene AR and Familial prostate cancer.