To study the behavior of cells deficient in NDRG1, we first examined the expression of NDRG1 in a panel of prostate cancer cell lines (DU145, LNCaP, and PC3 cells), normal immortalized prostate cells (RWPE and 957E/hTERT cells), and nontumorigenic HEK293 cells. This evidence concerns the gene NDRG1 and prostate carcinoma.