Hence, we examined the effect of SMYD2‐mediated methylation of ALK on the phosphorylation status of EML4‐ALK fused protein using two NSCLC cell lines, NCI‐H2228 and NCI‐H3122, both of which express EML4‐ALK fused protein and also express high levels of SMYD2 protein endogenously. This evidence concerns the gene ALK and non-small cell lung carcinoma.