Among those most validated diagnostic markers are SFRP2,20WNT221 and WIF1. 22 A few other studies have also found methylation-based biomarkers for predicting progression in CRC, which includes Myopodin,23KISS1,24hMLH1,25HPP1 and HLTF. 26, 27, 28 However, most studies lack independent validation. This evidence concerns the gene HLTF and colorectal carcinoma.