In this TDCC assay, the effector cells were spleen cells of human CD3 EDG–replaced mice and the target cells were Hepa1-6/hGPC3/HER2 cells, in which human glypican-3 (GPC3) and erb-b2 receptor tyrosine kinase 2 (HER2) are significantly expressed in Hepa1-6 mouse hepatocellular carcinoma (Fig. 8A and B). This evidence concerns the gene ERBB2 and hepatocellular carcinoma.