LEP and obesity due to melanocortin 4 receptor deficiency: We measured methylation in 3–4 CpG sites in the promoter regions of three candidate genes selected on the basis of different biological pathways that are involved in obesity: leptin (LEP, associated with appetite and fat metabolism [13]), corticotropin-releasing hormone (CRH, stress and appetite [14]), and intracellular adhesion molecule (ICAM-1, inflammation [15]).