In vitro co-culturing of each combination of cells such as CACs (HepG2 and SNU449)–ECs (HUVECs) and CACs–HSCs (LX2) as well as suppression of in vivo tumor growth and metastasis to lung in nude mice revealed that this miRNA regulated multiple aspects of HCC such as angiogenesis, invasion and metastasis by targeting three important pathways VEGF–VEGFR signaling, HGF–cMET signaling and ECM remodeling. The gene discussed is KDR; the disease is hepatocellular carcinoma.