ASS1-KO endometrial cancer cells generated by the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated 9 (CRISPR/Cas9) system showed enhanced cell sensitivity to arginine and resulted in increased cell motility and invasion capability in response to arginine following arginine starvation. Here, ASS1 is linked to endometrial cancer.