To address whether CXCR3+ monocytes/macrophages mediate melanoma engraftment in the lung, we first evaluated melanoma engraftment in WT and CXCR3−/− mice following the depletion of circulating monocytes and macrophages using clodronate liposomes (L-clodronate)28 prior to tumor inoculation. This evidence concerns the gene CXCR3 and melanoma.