Here, we report the discovery of three new loci associated with FECD, KANK4 (KN motif- and ankyrin repeat domain-containing protein 4), LAMC1 (laminin gamma-1) and LINC009970/ATPB1 (Na+, K+ transporting ATPase, beta-1 polypeptide), and confirm the strong effect of TCF4 variants in our large samples of FECD cases and controls. This evidence concerns the gene KANK4 and Fuchs endothelial corneal dystrophy.