Although the overexpression of LIMK1 and DADS had no effect on the expression of total cofilin1 protein, DADS could inhibit the phosphorylation of cofilin1 protein in colon cancer cells, whereas the overexpression of LIMK1 significantly promoted the phosphorylation of cofilin1 protein and antagonized cofilin1 dephosphorylation by DADS. Here, LIMK1 is linked to malignant colon neoplasm.