In keeping with these, we found that the feeding c57BL/6J mice with HFD for 10 weeks resulted in significant increases in the liver weight, hepatic fat deposition, liver and serum contents of TG and TC, and oxidized hepatic lipid content, as well as the activities of hepatic GOT and GPT, the key characteristics of NAFLD. Here, GPT is linked to metabolic dysfunction-associated steatotic liver disease.