CD40LG and microcephaly: A study in the State of Paraiba, Brazil, further illustrated the temporality where zika virus was detected in amniotic fluid in two pregnant women with fetal microcephaly.25 Analysis of amniotic fluid of the first 31 microcephaly cases in Pernambuco, Brazil, showed the presence of IgM specific antibody for zika virus in the 30 cases, indicating the association of microcephaly with zika virus infection in pregnancy.26