If this cobalt binds to albumin, it is likely to bind predominantly at site A and/or site B. In other words, the ACB assay measures largely the level of binding of cobalt at site A and/or site B, rather than at the N-terminus; therefore, ischemia-induced N-terminal modification (if present) would lead to only an insignificant effect (or possibly no effect at all) on the total albumin cobalt binding capacity. This evidence concerns the gene ALB and ischemia.