This γδ T-cell-mediated sensitisation of tumour cells to CD8+ T-cell killing was particularly striking when observed in real time using video microscopy, revealing an increased and more persistent calcium flux in CD8+ T cells in response to sensitised CSC-like cells (Figure 5e) that resulted in substantial target killing (Figure 5f; Supplementary Movies S1–S3). This evidence concerns the gene CD8A and neoplasm.