At 16 and 48 h post infection (p.i.), increasing amounts of HCoV-229E RNA (as assessed by RT-PCR using primer pairs specific for the nsp8 and spike protein coding regions, respectively) were detectable in HCoV-229E-infected cells, but not in control cells inoculated with heat- or UV-inactivated HCoV-229E (S1A Fig). The gene discussed is CHMP5; the disease is infection.