Correlation analysis of the expression levels of Annexin A1, Vimentin and S100A9 in different differentiation levels(CNE1,CNE2) and in different metastatic potential(5-8F,6-10B) cells indicated that Annexin A1 expression was negatively correlated with the expression of Vimentin and S100A9, and its down-regulation may cause up-regulation of Vimentin and S100A9, which may be closely related to the metastasis of NPC. The gene discussed is VIM; the disease is nasopharyngeal carcinoma.