ABCB4 and hepatocellular carcinoma: To do this, we analyzed DNA methylation data, obtained by methylated DNA immunoprecipitation (MeDIP) followed by array hybridization, from the late precancerous stages of HCC development in 12-month-old Mdr2/Abcb4-knockout (Mdr2-KO) male FVB strain mice, a well characterised model of chronic inflammation-mediated HCC [51, 52].