Knock-down of Sptr using the broad dopaminergic (TH-GAL4) and serotonergic (Trh-GAL4) drivers as well as the “obesity blocking neuron” driver (c673a-GAL4) [24], the insulin-producing cell driver (dilp2-GAL4), the oenocyte driver (OK72-GAL4), and the feeding-related peptidergic neuron drivers sNPF-GAL4 and AstA-GAL4 do not affect feeding amount (S8C–S8I Fig). This evidence concerns the gene TRH and Obesity.