As Fig 6A–6E revealed, the expression levels of KRT23, MMP7, STRA6, WNT2, GRHL3 were significantly up-regulated in READ tissues; moreover, the expression levels of DPP6, CA7, BAI3, FIGF, RSPO2, SORCS1, PDZRN4, LONRF2, CNGA3, HAND2 and GNAO1 were obviously down-regulated in READ tissues compared with matched mucosa tissues based on the microarray analyses (Fig 6F–6P). Here, RSPO2 is linked to reading.