9 candidate genes were randomly selected for qRT-PCR examination, ATP2B4, NR3C1, ROR1 and PPKCB were down-regulated in READ, TCF7, SLC6A6, PDPN, WNT2 and ONECUT2 were up-regulated in READ compared to paired adjacent non-tumor tissues, which were accordance with our analyses. This evidence concerns the gene TCF7 and reading.