Thus, to dissect the role of Sdc1 in colitis-related tumorigenesis, a single dose of AOM was injected to 10–12 week-old Sdc1-KO mice and their wild-type (wt) counterparts prior to 3 cycles of DSS treatment (as indicated in Fig 1A, and described in 'Methods'). The gene discussed is SDC1; the disease is colitis.