NEDD4 and hepatocellular carcinoma: To determine whether Nedd4 plays a role in other human cell lines that are susceptible to JEV infection, we assessed the effect of Nedd4 depletion on JEV infection of human neuroblastoma SH-SY5Y cells, human umbilical vein endothelial cells (HUVECs) and human hepatocellular carcinoma Huh7 cells.