In addition, whilst previous studies had shown that TSG101 was also required for infection with BPV-1 PsVs30 here we show an important role for both TSG101 and VPS4 for infection with cutaneous HPV-5 PsVs and also mouse MmuPV-1 and rabbit SfPV-1 indicating that the ESCRT machinery most likely plays a role in infection with multiple PV types, and demonstrates an evolutionarily conserved step in the infectious entry of multiple PV types. This evidence concerns the gene TSG101 and acquired polycythemia vera.