As shown in Figures 8D–F, compared with the control group, miR-590-3p, EMAP-II + TMZ or miR-590-3p + EMAP-II + TMZ significantly up-regulated LC3-II and Beclin-1 protein expression and down-regulated p62/SQSTM1 protein expression in tumor tissues. The gene discussed is BECN1; the disease is neoplasm.