We found that the expression level of miR-590-3p in tumor tissues were up-regulated in miR-590-3p + EMAP-II + TMZ group compared with the miR-590-3p group or EMAP-II + TMZ group, besides, miR-590-3p + EMAP-II + TMZ significantly up-regulated LC3-II and Beclin-1 protein expression and down-regulated p62/SQSTM1 protein expression in tumor tissues compared with the miR-590-3p group or EMAP-II + TMZ group. This evidence concerns the gene SQSTM1 and neoplasm.