TWIST1 and breast carcinoma: Since increased migration is considered a feature of EMT49, we determined if the loss of WT1 also affects the epithelial/mesenchymal balance of breast cancer cells and analysed the RNA levels of several EMT drivers (SNAI1, SNAI2, ZEB1, ZEB2, TWIST1), epithelial markers (CTNNA1, CTNNA2, CTNND1, CDH1, KRT18) and mesenchymal markers (VIM, TNC, TGFB1, CDH2, MMP2, MMP9) through quantitative real-time PCR.