To understand the TNF-induced signal changes in epithelial HF stem cells, we performed iTRAQ analysis; the result shows TNF induces AKT phosphorylation (p-AKT) in epidermal stem cells (Epi-SCs) and Lgr5+ cells, and AKT signals positively regulate β-catenin signalling. The gene discussed is AKT1; the disease is hydrops fetalis.