TNF and Hand-foot syndrome: To further verify the contribution of macrophage-derived TNF, we employed Lysmcre/+:TNFflox/flox mice35, in which macrophages and neutrophils do not express TNFA. Wounding was associated with a significantly (P<0.005) lower number of anagen HFs in these mice compared with the numbers seen in their littermate controls (Fig. 2i,j).