Dot blotting showed the release of HMGB1 from cells treated with EnAd and Ad11p started at 3 days post infection (POI), reaching a maximum at day 3.5–4 (Figure 6D), whereas Ad5-treated cells gave only a weak signal even at day 4, when death of most cells was verified by MTS and xCELLigence (Figures 1B–1E). The gene discussed is HMGB1; the disease is infection.