To confirm that our method is applicable to the research of MNDs, we generated human iPSCs from the fibroblasts of a familial ALS patient with mutant superoxide dismutase 1 (SOD1 ALS) by transducing the four transcription factors Oct3/4, Sox2, Klf4, and c-Myc, as previously reported3, 7 (Table S4). This evidence concerns the gene KLF4 and amyotrophic lateral sclerosis.