Therefore, to determine whether this was also the case for our cohort, we performed two multi-color immunofluorescence stainings on consecutive whole slides of a highly infiltrated POLE-mutant tumor sample using the following combinations of monoclonal antibodies: CD68–CD163 – epithelial cell marker cytokeratin, and PD-L1–PD-1, respectively (Fig. 4). This evidence concerns the gene PDCD1 and neoplasm.