CCND1 and breast adenocarcinoma: To verify the importance of cyclin D1 for the activity of LCAHA, we tested an additional three cell lines: breast adenocarcinoma cell line MCF-7 (cyclin D1 dependent [Grillo et al., 2006]), osteosarcoma cell line U-2 OS (cyclin D1 positive), and osteosarcoma cell line SAOS-2 (cyclin D1 negative).