Next, reporter constructs containing either the wild-type (WT) HMGN5 3′-UTR or HMGN5 3′-UTR with mutation (MT) at the predicted miR-140-5p target sequence were cotransfected into osteosarcoma U2-OS cells and then transduction of Mimic NC, miR-140-5p mimics, anti-NC or anti-miR-140-5p. Here, HMGN5 is linked to osteosarcoma.