Inhibiting Notch 2 decreased HCC cell proliferation (Ortica et al., 2014[29]) and tumour burden (Huntzicker et al., 2015[18]), while Notch 1 and 2 overexpression in the liver resulted in spontaneous HCC development with biliary or LPC characteristics (Villanueva et al., 2012[35]; Dill et al., 2013[8]; Zender et al., 2013[40]). Here, NOTCH1 is linked to hepatocellular carcinoma.